Amplicons for NGS were prepared following cloning of PCR fragments into mammalian expression vectors as outlined in Fig 8A. The average number of full-length reads was determined based on the percenta
(a) Accession number of genes found in Fragaria vesca genome (http://www.strawberrygenome.org/andhttp://www.rosaceae.org/) that may be amplified by the designed primer pairs. (b) Theoretical melting t