Chronic oral administration of nicotine affects transcriptomic profiling in conditional Presenilin 1/2 knockout mice and wild type mice

we found that chronic oral (3 months) administration of nicotine had different effects on transcriptomic profiling in prefrontal forebrain cortex of wildtype (WT) and Presenilin 1/2 double knockout mice. 8-month-old Presenilin 1/2 double knockout mice (DKO) and the wildtype mice (WT) were exposed to nicotine by drinking water at the dose of 100 ug/ml (DKO-N and WT-N, respectively) or water (DKO-B and WT-B, respectively) ad libitum for 3 months, after the behavior tests, the prefrontal forebrain cortex of the four groups of mice were collected for RNA-seq.我们发现，经3个月慢性口服给药后，尼古丁对野生型（wildtype, WT）与早老素1/2双敲除（Presenilin 1/2 double knockout, DKO）小鼠的前额叶皮层转录组谱（transcriptomic profiling）产生了不同影响。本实验选取8月龄的早老素1/2双敲除小鼠及野生型小鼠，通过自由饮用含100 μg/ml尼古丁的饮用水（分别对应DKO-N组与WT-N组）或纯水（分别对应DKO-B组与WT-B组）的方式进行为期3个月的暴露处理；待行为学测试结束后，采集四组小鼠的前额叶皮层组织用于RNA测序（RNA-seq）。