Dose-dependent consequences of sub-chronic fentanyl exposure on neuron and glial co-cultures

It remains poorly understood what and how changes in brain activity from chronic fentanyl use influences the respective behavioral outcome. We examined the functional and molecular changes to cortical neural network activity following sub-chronic exposure to two fentanyl concentrations, a low (0.01 µM) and high (10 µM) dose from primary rat co-cultures, containing cortical neurons, astrocytes, and oligodendrocyte-lineage cell. Overall design: Primary rat co-cultures, containing cortical neurons, astrocytes, and oligodendrocyte precursor cells, at 24-26 days in vitro were treated with a low (0.01 µM) or high (10 µM) dose for four days before RNA was extracted.