Single cell RNA-Seq of sorted nephron progenitor cells from pooled mouse kidneys

Kidneys from multiple litters of Six2GFP+ E14.5 mouse embryos were pooled into three replicate tubes and dissociated in parallel. Six2GFP+ cells were isolated and processed for single cell sequencing using 10x Genomics technology. This resulted in a dataset of 7844 single cells representing the nephron progenitor population of the mouse kidney. High resolution single cell analysis of the nephron progenitor population in Six2GFP+ mice.将多窝Six2GFP阳性（Six2GFP+）E14.5小鼠胚胎的肾脏组织混合后均分至三个重复实验管中，并同步进行组织解离。分离得到Six2GFP阳性细胞后，采用10x基因组学（10x Genomics）技术开展单细胞测序实验，最终获得包含7844个单细胞的数据集，该数据集涵盖小鼠肾脏的肾单位祖细胞群体。本数据集针对Six2GFP阳性小鼠体内的肾单位祖细胞群体开展了高分辨率单细胞分析。