Whole genome bisulfite sequencing of VANC1 expressed Arabidopsis thaliana

VANC protein which is encoded in VANDAL transposons have sequence-specific anti-silencing activity. So far, active VANC21 and VANC6 were identified, and when their genomic sequences are transformed into wild-type Arabidopsis plants, VANDAL21 and VANDAL6 families respectively are specifically hypomethylated. VANC proteins localize in non-coding regions within target VANDALs, and subfamilies-specific short motifs are accumulated. Specific binding between short motif and VANC protein provides specific anti-silencing systems in VANDAL transposons. We newly identified active VANC1, which have evolutionary ancestral domains structure, and analyzed their effects on DNA methylation status by WGBS.