Full-length uncropped Western blots
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Uncropped Western blot raw data for USP7, GPX4, and GAPDH (loading control) in [BCPAP, K1, 8305C, 8505C]. Cellular extracts were resolved by SDS-PAGE, transferred to PVDF membranes (Roche #03010040001), and probed with anti-USP7 (CST #4833, 1:1000), anti-GPX4 (Proteintech #67763, 1:5000), and anti-GAPDH (Proteintech #60004, 1:10000). Secondary detection used HRP-conjugated antibodies (1:5000) and Immobilon ECL substrate (Millipore #WBKLS0500). Images were captured with [instrument] and include all lanes and molecular weight markers. Data represent [n] independent replicates.
