Zea mays Genome sequencing. Zea mays
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下载链接：
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA412222
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资源简介：
B73 seedling roots were pulse-labeled with the thymidine analog 5-Ethynyl-2'-deoxyuridine (EdU) for 1 h to allow actively replicating DNA to incorporate the EdU. The terminal 0-3 mm root segments were excised, fixed in 1% formaldehyde for 15 minutes, washed in PBS, and snap frozen. Nuclei were isolated from the frozen, excised roots, the incorporated EdU was "clicked" to Alexa Fluor-488 (AF-488), and total DNA was stained with DAPI. Nuclei were analyzed based on EdU incorporation (AF-488 fluorescence) and DNA content (DAPI fluorescence), and 2C and 8C unlabeled nuclei were sorted. Genomic DNA was extracted from sorted nuclei and Illumina sequenced.
创建时间：
2017-09-26
