Reactivation of Tumor Suppressor in Breast Cancer by Enhancer Switching through NamiRNA Network. Reactivation of Tumor Suppressor in Breast Cancer by Enhancer Switching through NamiRNA Network

We reported that low expression of miRNA in cancer as a recognized signature leads to loss function of TSGs in breast cancer. In 157 paired breast cancer and adjacent normal samples, tumour suppressor gene GPER1 and miR-339 are both downregulated in Luminal A/B and Triple Negative Breast Cancer subtypes. Mechanistic investigations revealed that that miR-339 upregulates GPER1 expression in breast cancer cells by switching on the GPER1 enhancer, which can be blocked by enhancer deletion through the CRISPR/Cas9 system. Overall design: 1). Examination of H3K27ac histone modification alterations in 293T cells after overexpressing miR-339; 2). mRNA profiles of 4175 cells after overexpressing miR-339