Sonoreactor-Based Technology for Fast High-Throughput Proteolytic Digestion of Proteins

Fast (120 s) and high-throughput (more than six
samples at once) in-gel trypsin digestion of proteins using
sonoreactor technology has been achieved. Successful
protein identification was done by matrix-assisted laser
desorption/ionization time-of-flight mass spectrometry,
MALDI-TOF-MS. Specific identification of the adenylylsulphate reductase alfa subunit from a complex protein
mixture from Desulfovibrio desulfuricans ATCC 27774 was
done as a proof of the methodology. The new sample
treatment is of easy implementation, saves time and
money, and can be adapted to online procedures and
robotic platforms.
Keywords: Protein digestion · sonoreactor · MALDI-TOF-MS