Identification of <i>VdCYC8</i>-complemented mutants in <i>Verticillium dahliae</i> by PCR identification and <i>VdCYC8</i> relative expression analysis.
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A) Confirmation of the presence of the wild-type complemented VdCYC8 fragment. B) Confirmation of the presence of the chlorimuron cassette in the ΔCYC8-C strains. Lanes 1–15 indicate PCR results using primer Sul-F/R and DNA template obtained from the different complemented mutant strains, respectively; Lane 16 represents T286. C) Relative expression analysis of VdCYC8 using primer CYC8RT-F/R. *Indicates a significant difference between mutant and the wild-type strain Vd080 (P<0.05).
创建时间：
2015-12-03
