scRNA seq analysis on immune cells isolated from nGD mice brain

We observed significant changes in gene expression between Wt and nGD brain. nGD brains are highly enriched for immune infiltrates, both microglia and immune infiltrates are activated in nGD brains. Through scRNA seq we identified that, microglia specific Gba deletion lead to higher activation and expression of DAM signature genes compared to control mice. The aim of the study is to identify immune microenvironment of of GD associated neuronopathy using single cell RNAseq technology. We developed mouse models that lack Gba gene in individual cell types with in the brain and evaluated gene expression changes using 10X scRNA seq.