Expression data from purified control and Brdt-delta-BD1 round spermatids

Brdt is a testis specific member of a family of chromatin interacting proteins. All of the family members have been shown to regulate transcription. Brdt is highly expressed in round spermatids, and may play a role in transcriptional regulation in these cells. We investigated transcriptional changes in mutant round spermatids that were homozygous for a mutation in which the first bromodomain of Brdt was removed. Round spermatids were purified from seven adult animals of each genotype for each Affymetrix microarray. Purity of round spermatids was assessed by propidium iodide staining.Brdt是染色质互作蛋白家族的睾丸特异性成员。该家族所有成员均已被证实可调控转录。Brdt在圆形精子细胞（round spermatids）中呈高表达，可能在这类细胞的转录调控中发挥作用。我们对携带Brdt首个溴结构域（bromodomain）缺失纯合突变的突变型圆形精子细胞的转录变化进行了研究。针对每一块Affymetrix微阵列（Affymetrix microarray），我们从对应基因型的7只成年动物体内纯化得到圆形精子细胞，并通过碘化丙啶染色（propidium iodide staining）评估其纯度。