Effect of GM-CSF priming on TNF-α production by BMDM in response to bacterial stimulusa.

aBMDM from WT (C57Bl/6) or deficient mice (−/−) for TLR2, TLR1, TLR6, TLR4, CD14 and MyD88 primed with GM-CSF, were incubated with AraLAM (5000 ng/mL), LM (5000 ng/mL), Malp2 (300 ng/mL), Pam3-CSK4 (5000 ng/mL) or LPS (500 ng/mL) for 24 h in vitro. TNF-α was measured by ELISA. Percentage of inhibition of TNF-α production in deficient mice compared to WT.bp, significantly versus WT production. Data are mean ± S.D, n = 6 mice per genotype from two independent experiments.cNegative values corresponds an increment in correlation with WT production.