MeRIP-seq analysis of replicative senescent MEFs and WI-38 cells

Cellular senescence is the most intuitive manifestation of individual senescence, and m6A is one of the most common modifications in mRNA, playing an important role in regulating gene expression, splicing, RNA stability, and mRNA degradation. Previous research has suggested that m6A levels play an important role in the occurrence and development of aging and aging-related diseases. However, there are few reports on the role of m6A in cellular senescence. Here, we conducted combined MeRIP-seq and RNA-Seq analyses on non-senescent and senescent MEFs and WI-38 cells. The results revealed substantial disparities in the distribution of m6A sites across the transcriptome between senescent and non-senescent cells. We then performed RNA-seq (also as input) and MeRIP-seq analysis using non-senescent and replicative senescence MEFs and WI-38 cells.