Figure 4 - tracking nuclei across tissue layers

Nuclear tracks in both muscle and endodermal layers extracted from 2D pullback representation of the w, hand-GAL4; UAS-hand-GFP; klar / histone:GFP embryo. <br> relative_motion_tracks.mat contains a full summary of the data, with tracks from each layer stored in a struct. <br> relative_motion_individual_curves_raw.mat contains a struct with fields: timestamps : timestamp of each image in hours duG : geodesic distance along the tissue surface from a given nucleus in the muscle layer to its partner nucleus in the endodermal layer. The partner is defined as the closest endoderm nucleus at time t=0. euclideanDistanceTraveledU : Euclidean distance traveled, pathlength integrated over a suitably smoothed 3D trajectory from the initial time to the current timepoint. <br> endoderm_tracks_correction.mat stores a cell element for each tracked nucleus across the 60 timestep dataset. Here track positions are given in 2D pulllback pixel space (see accompanying dataset). The third column of the data maps to a graph-based indexing system used for tracking. Many nodes are marked as zero, indicating they were manually corrected after the graph-based system finished its computation. <br> muscle_tracks.mat stores a cell element for each tracked nucleus across the 60 timestep dataset. Here track positions are given in 2D pulllback pixel space (see accompanying dataset). The third column of the data maps to a graph-based indexing system used for tracking. Many nodes are marked as zero, indicating they were manually corrected after the graph-based system finished its computation. <br> <br> <br> <br>