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Real-time quantitative PCR analysis of mRNA from the colon of WT, Nod2-/-, Pglyrp3-/-, and Pglyrp3-/-Nod2-/- mice treated with oral DSS. Mus musculus

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下载链接:
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA206429
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资源简介:
Wild type (WT), Nod2-/-, Pglyrp3-/-, and Pglyrp3-/-Nod2-/- mice (BALB/c) were treated with oral 5% dextran sodium sulfate (DSS) for 48, 72, or 96 hrs, their colons were removed and homogenized, and RNA was isolated using the TRIZOL method. Quantitative reverse transcription real-time PCR (qRT-PCR) was used to quantify the amounts of mRNA in the colon using the inflammatory gene expression RT2 Profiler PCR Array from Qiagen/SA Biosciences. Overall design: qRT-PCR gene expression profiling
创建时间:
2013-06-03
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