Differential Gene expression of C. elegans larvae following tunicamycin treatment in the absence of manf-1

Microarray analysis was performed to evaluate the changes in gene expression that are associated with manf-1 deficiency with and without exposure to tunicamycin. RNA was isolated from four groups, (wildtype (N2) and manf-1 mutant (tm3603) L1 stage larve with and without 5 hours exposure to tunicmycin (5microgram/milliliter)) for transcriptomic profiles analysis Identification of genes expressed after tunicamycin treatment in two groups (wildtype and manf-1 mutant) with three replications per group.