ChIP-seq of bovine cells (MDBK) to study butyrate-induced histone modification

Utilizing next-generation sequencing technology, combined with ChIP (Chromatin Immunoprecipitation) technology to analyze histone modification (acetylation) induced by butyrate and to map the epigenomic landscape of normal histone H3, H4 Overall design: Cells were treated with 10 mM butyrate for 24 hr, The cells were scraped from the flask and homogenized with ice-cold Dounce homogenizer to release the nuclei. The collected nuclei were resuspended in digestion buffer and enzymatic shearing was performed. ChIP with anti H3, H4 and acetyl-H3 and acetyl-H4.

采用下一代测序技术与ChIP（染色质免疫沉淀）技术相结合，以分析丁酸诱导的组蛋白修饰（乙酰化）并绘制正常组蛋白H3、H4的表观基因组图谱。整体设计：将细胞用10 mM丁酸处理24小时，随后从培养瓶中刮取细胞，并与冰冷的Dounce均质器混合均质，以释放细胞核。收集到的细胞核悬浮于消化缓冲液中，并进行酶切剪切。使用抗H3、H4和乙酰化-H3及乙酰化-H4进行ChIP实验。