Wound memory of epidermal stem cells [scRNAseq L1 PRT]

Wound priming in epidermal Lrig1 stem cell progeny leads to a more efficient response to future injuries. To identify a key epigenetic factor of this cellular adaptation, we performed an epigenetic drug screening in Lrig1 genetically labled mice. Pre-treatment with PRT4165 lead to a more efficient wound closure. To understand if PRT4165 was able to recapitulate wound priming, we performed single cell RNA-seq transcriptome profiling of epidermal Lrig1 stem cell progenies from PRT4165-treated or untreated murine skin. [PRT4165is an inhibitor of the enzymatic activity of Ring1a/Ring1b, component of the Polycomb repressive complexes 1 (PRC1), responsible for the monoubiquitination of lysin 119 on histone H2A (H2AK119ub)] Overall design: Single cell RNA-seq transcriptome profiling of epidermal Lrig1 stem cell progenies. After genetical labeling through tamoxifen application, tomato positive epidermal cells were sorted from treated with PRT4165 or vehicle treated tail skin.