Supporting data for "An effective strategy for assembling the sex-limited chromosome"

Most available reference genomes lack the sequence map of sex-limited (such as Y and W) chromosomes, which make the assemblies incomplete and difficult to conduct further research on sex chromosomes. Recent advances in long reads sequencing and population sequencing raise the opportunity to assemble sex-limited chromosomes without the traditional complicated experimental efforts. We introduced the first computational method, Sorting long Reads of Y or other sex-limited chromosome (SRY), which achieved improved assembly results compared to flow sorting. Specifically, SRY outperformed in the heterochromatic region and demonstrated comparable performance in other regions. Furthermore, we observed that SRY enhanced the capabilities of the hybrid assembly software, as evidenced by improved continuity and accuracy. Our method can realize true complete genome assembly and facilitate downstream research of sex-limited chromosomes.