Aggregation vs. nonaggregation strategies in Dictyostelium discoideum amoebae in response to starvation stress: raw data

Spreadsheet containing the data shown in Figure 2A.Spreadsheet containing the data shown in Figure 2B.Spreadsheet containing the data shown in Figure 2C.Spreadsheet containing the data shown in Figure 3A.Spreadsheet containing the data shown in Figure 3B.Spreadsheet containing the data shown in Figure 3C.Spreadsheet containing the data shown in Figure 3E.Spreadsheet containing the data shown in Figure 3F.Spreadsheet containing the data shown in Figure 4D.Spreadsheet containing the data shown in Sup. Figure S1.Spreadsheet containing the data shown in Sup. Figure S4.Spreadsheet containing the data shown in Sup. Figure S5.Macros is an archive containing the set of ImageJ macros used for image analysis that was used to produce the data shown in Figures 2, 3, 4, S1, S4 and S5.Code is the simulation code used in Figure 6.
Sup. Movie S1. Partitioning of Dictyostelium populations under starvation stress into aggregating vs non-aggregating cells. 0.25% or RFP-expressing AX3 cells mixed with 99.75% of GFP-expressing cells were plated according to the standard “sudden” starvation experimental protocol. After aggregates form, most single RFP-expressing cells are found in aggregates, and a minority of them are found outside of aggregates even though aggregating and non-aggregating cells were intermixed at the onset of starvation. Both phase contrast(left) and red fluorescent (right) images show that non-aggregating cells are alive and motile. Counting single RFP-cells before aggregation and cells that are outside of aggregates after aggregation provides a direct estimate of aggregating and non-aggregating cell numbers.
Sup. Movie S2. Non-aggregating cells have not stably lost the ability to aggregate. After the aggregation had finished and fruiting bodies started to form, fresh nutrients (dead bacteria) were added to the area with non-aggregating cells. Once bacteria have been consumed these cells aggregate and develop into a fruiting body.
Sup. Movie S3. Non-aggregating cells are capable of resuming growth immediately upon food arrival while aggregating cells are embedded in development. 18h after plating cells on nutrient-free agar, aggregating cells have formed slugs while non-aggregating cells are starving. Nutrition in form of dead bacteria was added at this point. Multicellulardevelopment goes on until the formation of fruiting bodies because cells in aggregates are irreversibly committed to development even in the presence of food. In contrast, nonaggregating cells feed on bacteria and divide several times. At the end of fruiting body formation, non-aggregating cells have already consumed most of the bacteria.

包含图2A所示数据的电子表格。包含图2B所示数据的电子表格。包含图2C所示数据的电子表格。包含图3A所示数据的电子表格。包含图3B所示数据的电子表格。包含图3C所示数据的电子表格。包含图3E所示数据的电子表格。包含图3F所示数据的电子表格。包含图4D所示数据的电子表格。包含补充图S1所示数据的电子表格。包含补充图S4所示数据的电子表格。包含补充图S5所示数据的电子表格。Macros 是一个存档，其中包含了用于图像分析的 ImageJ 宏集，这些宏被用于生成图2、3、4、S1、S4和S5中的数据。Code 是用于图6的模拟代码。补充电影S1。在饥饿胁迫下，群体 Dictyostelium 在聚集细胞与不聚集细胞之间的分区。0.25% 或表达RFP的AX3细胞与99.75%的表达GFP细胞按照标准的“突然”饥饿实验方案混合接种。在聚集形成后，大多数单个表达RFP的细胞位于聚集体内，尽管在饥饿开始时聚集细胞和不聚集细胞是混合的，但其中少数细胞位于聚集体外。相衬（左）和红色荧光（右）图像显示，不聚集细胞存活且具有运动能力。在聚集前计数单个RFP细胞以及在聚集后聚集体外的细胞数量，可以直接估计聚集细胞和不聚集细胞数量。补充电影S2。不聚集细胞并未稳定丧失聚集的能力。在聚集完成并且子实体开始形成后，向不聚集细胞的区域添加了新鲜的营养物质（死亡的细菌）。一旦细菌被消耗，这些细胞就会聚集并发育成子实体。补充电影S3。不聚集细胞能够在食物到达后立即恢复生长，而聚集细胞则被嵌入发育过程中。在将细胞接种于无营养琼脂18小时后，聚集细胞已经形成了蠕虫状结构，而不聚集细胞正处于饥饿状态。此时添加了以死亡细菌形式存在的营养物质。多细胞发育一直持续到子实体的形成，因为即使在有食物存在的情况下，聚集体内的细胞也因不可逆地致力于发育而不会改变。相反，不聚集细胞以细菌为食并分裂多次。在子实体形成结束时，不聚集细胞已经消耗了大部分细菌。