Gene expression signatures after immunisation with Boostrix - alum adsorbed tetanus toxoid, reduced diphtheria toxoid and acellular pertussis vaccine (BIOVACSAFE protocol 305D2). Gene expression signatures after immunisation with Boostrix - alum adsorbed tetanus toxoid, reduced diphtheria toxoid and acellular pertussis vaccine (BIOVACSAFE protocol 305D2)

The time course of whole blood gene expression was determined in healthy adult human participants who were randomised to receive either a single intramuscular immunisation with Boostrix vaccine (characteristics: treatment = BOOSTRIX) or equivalent volume saline control (characteristics: treatment = PLACEBOD2). Participants are identified who reported having had an immunisation with Boostrix within the past 5 years (characteristics: recent boostrix = 1). Overall design: Participants attended a single outpatient unit (CEVAC, Gent, Belgium). Pre-immunisation baseline samples were drawn on day 0, and further samples collected on days 1, 7 and 28. Half the participants were allocated to also attend on day 2 whereas the other half attended on day 3 instead. All samples were collected into PaxGene tubes. Immunisations were given on day 0 after baseline blood draw.