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Proteomics data (processed) for macrocycle-based PROTACs targeting cyclophilin A

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B2FIND2026-04-29 收录
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THP-1 Dual cells were treated with 5 µM Cyp inhibitor or DMSO for 72 hrs. After washing with PBS, cells were lysed (5 % SDS, 5 mM tris(2-carboxyethyl)phosphine (TCEP), 10 mM...

将THP-1双细胞(THP-1 Dual cells)以5 µM的Cyp抑制剂(Cyp inhibitor)或二甲基亚砜(DMSO)处理72小时。经磷酸盐缓冲液(PBS)洗涤后,使用含5%十二烷基硫酸钠(SDS)、5 mM三(2-羧乙基)膦(TCEP)、10 mM……的裂解液对细胞进行裂解。

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