Klebsiella pneumoniae strain:MRK9 Genome sequencing. Klebsiella pneumoniae strain:MRK9

Nosocomial and community acquired infections caused by multidrug resistant (MDR) Gram-negative bacteria (GNB), represent a growing problem worldwide. These organisms are responsible for a spectrum of infections; ranging from urinary tract infections (UTI), meningitis, septicaemia, pneumonia, to wound and device-associated infections. Polymyxins (polymyxin B and colistin) are increasingly used as antibiotic of last resort, however, emergence of polymyxin resistance (PR) is increasingly observed among bacterial pathogens. Polymyxin resistance is mediated by increased expression of efflux pumps, capsule formation and alterations in LPS of bacterial outer membrane. In recent years emergence of plasmid mediated resistance due to mcr-genes poses a threat for the rapid global spread. Analysis of the factors responsible for decreased susceptibility to polymyxin, is believed to enhance understanding of antibiotic resistance and in identifying novel markers of polymyxin resistance for rapid identification of resistance in healthcare settings. In this study, we screened and characterized the colistin resistant bacteria. We also aimed to identify the resistant determinants of colistin in the resistant isolates.