Transcriptomic profile of ovarian cancer stem cells dervived from ascites of chickens.

The objective of the present study was to determine the trancriptomic profile of ovarain cancer stem cells (CSCs) dervived from acites of chickens. Ovarian CSCs were sorted based on presence of a stem cell marker; aldehyde dehydrogenase (ALDH) and then subjected to RNASEq. Pairwise statistical analysis of transcriptomic data using Deseq2 software revealed 1800 differentially expressed (DEGs) in ALDH+ cells as compared to ALDH- cells Overall design: Ovarian CSCs were seggregated based on the presence of ALDH activity and then sequenced. We performed RNAseq analysis using the Illumina® software platform on RNA samples extracted from ALDH+ and ALDH- cells. On average, 50 million 125 base pair paired-end reads were sequenced for each replicate. Statistical analysis was performed for pairwise comparison between treatment and control group.