Nutrient sensing mediated by O-GlcNAcylation regulates gene expression through epigenetic mechanisms during embryonic neurogenesis

We show that pharmacological upregulation of O-GlcNAc levels during hESCs-neuronal differentiation leads to upregulation of key neurogenic TF gene which in turn is associated with increased H3K4me3 levels, reduction in H3K27me3 levels and increase in Pol IISer5 phosphorylation. Our results demonstrate O-GlcNAc mediated sensitivity of chromatin to nutrient status, and indicate how metabolic dysregulations could affect stem cell fate decisions through epigenetic gene regulatory mechanisms RNA-seq in four stages of hESC neural differentiation on samples treated with TMG and solvent control