An assessment of the role of 5′ and 3′ UTRs from select purine-responsive candidates on mRNA abundance and translational regulation.

L. donovani cell lines were generated in which a Fluc reporter was integrated in place of one allele of the indicated locus; each Fluc reporter line also contained an Rluc reporter integrated at the UMPS locus as an internal normalization control. Changes in Fluc activity and mRNA abundance (qRT-PCR Fluc), and mRNA abundance of the corresponding endogenous allele (qRT-PCR Gene) following 24 h purine starvation were determined in parallel from aliquots of the same culture. All qRT-PCR data were normalized to UMPS. The mean and standard deviation determined from two independent biological replicates is shown for each analysis.