DNA modifications in specific nuclei populations purified from human brain tissue

Epigenetic variation has been implicated in brain development and the progression of brain disorders including neuropsychiatric and neurodegenerative diseases. The human cortex is highly complex, comprising a mix of different neural cells. We sought to generate reference maps of DNA modifications in each cell type using a Fluorescence Activated Nuclei Sorting (FANS) method developed by our group to separate cell-specific nuclei from bulk cortex tissue using cell-specific markers. DNA from each nuclei population was isolated and sequenced using Oxford Nanopore technology. DNA sequence was base-called and base modification (5-methyl-cytosine and 5-hydroxymethyl-cytosine) detected. For purposes of comparison, 5-methyl-cytosine (5mC) and 5-hydroxymethyl-cytosine (5hmC) were also detected using duet multiomics solution evoC 6-base sequencing (https://biomodal.com/products/duet-evoc/). The tab-delimited data files contain percentage modification of cytosines (5mC and 5hmC) located in autosomes of hg38 human assembly where the coverage is >=10 in neuronal (NeuN) and oligodendrocyte (Sox10). Data is merged from three different individuals.