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Assembly Free Viral Genomes from Station ALOHA

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NIAID Data Ecosystem2026-04-25 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP189859
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We developed an assembly-free, single-molecule nanopore sequencing approach enabling direct recovery of high-quality viral genome sequences from environmental samples. Applied to samples from Station ALOHA in the North Pacific Subtropical Gyre, the method yielded full-length double-stranded DNA virus genome sequences and revealed viruses not fully recovered using short read assembly approaches in the same samples. Additionally, novel virus-like DNA sequences were discovered, whose structures, gene contents and concatemer lengths suggested they represent phage-inducible chromosomal islands packaged in discrete concatemeric lengths within phage particles.
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2020-05-29
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