File S1 - Chinese Wild-Growing Vitis amurensis ICE1 and ICE2 Encode MYC-Type bHLH Transcription Activators that Regulate Cold Tolerance in Arabidopsis

Contains the files: Figure S1 Multiple alignment of the mRNA sequences of ICE-homologous from different Vitis species. Identical nucleotide sequences are highlighted on a black background while white boxes indicate at least three identical nucleotides. The GenBank accession numbers are reported as follows: VaICE1 (KC815984), VaICE2 (KC815985), VaICE14 (HM231151), VvICE1 (JQ707298), VvICE1a (KC831748), VvICE1b (KC831749), VrICE1 (KF994961), VrICE2 (KF994962), VrICE3 (KF994963) and VrICE4 (KF994964). Figure S2 Protein sequence similarity and phylogenetic clustering of ICE from three different Vitis species. (A) Protein sequence alignment of 10 grapevine ICEs. Identical residues are outlined in black. Amino acids are numbered on the right. (B) Phylogenetic tree based on the deduced amino acid sequences of ICEs from three different Vitis genotypes. A maximum-likelihood phylogenetic tree of the amino acid sequences of ICE from V. amurensis (VaICE1, AGP04217; VaICE2, AGP04218; VaICE14, ADY17816), V. vinifera (VvICE1, AFI49627; VvICE1a, AGQ03810; VvICE1b, AGQ03811), and V. riparia (VrICE1, AGG34704; VrICE2, AIA58705; VrICE3, AIA58706; VrICE4, AIA58707) is constructed by MEGA 5.0 with 1000 bootstrap tests. The branch support values are indicated. The length of the scale bar corresponds to 0.05 substitutions per site. Table S1 Feature lists of BLAST results or queries of ten grapevine ICE genes available in FLAGdb++. A Blastp search with an E-Value of 1.E-50 was performed on V. vinifera using the ten grapevine ICE proteins from V. amurensis (VaICE1, AGP04217; VaICE2, AGP04218; VaICE14, ADY17816), V. vinifera (VvICE1, AFI49627; VvICE1a, AGQ03810; VvICE1b, AGQ03811), and V. riparia (VrICE1, AGG34704; VrICE2, AIA58705; VrICE3, AIA58706; VrICE4, AIA58707) as query. (DOC)