Transcriptional dysregulation impairs efficacy of neural stem cell activation during aging [ChIP-seq]

To get insight into the histone modification in the transcriptional dysregulation of NSC aging, we profiled the principal regulatory histone marks at the promoters and enhancers of age-dependent genes using ChIP-sequencing. Primary NSCs were isolated from the subventricular zone of the lateral ventricles (SVZ) in the brains of young and aged mice, respectively. The NSCs were maintained in the initial proliferation medium and used in ChIP-sequencing analysis.