Transcription profiling by array of olfactory epithelium from Atf5+/+ and Atf5-/- mice

We have found that the expression pattern of Atf5 is highly restricted to the olfactory system, strongly suggesting that Atf5 is an olfactory sensory neuron-specific transcription factor. To test this possiblity, we compared gene expression profiles in olfactory epithelium (septa and turbinates) from Atf5+/+ and Atf5-/- mice. Olfactory septa and turbinates were dissected from Atf5+/+ and Atf5-/- P0 pups from different litters (n=3 for each genotype) and pooled into a single sample. Three independent samples were prepared for each genotype.我们发现，Atf5的表达模式高度局限于嗅觉系统，强烈提示Atf5是一种嗅觉感觉神经元特异性转录因子。为验证这一可能性，我们对比了Atf5纯合野生型（Atf5+/+）与纯合敲除型（Atf5-/-）小鼠的嗅觉上皮（包括鼻中隔与鼻甲）中的基因表达谱。我们从不同窝的Atf5+/+与Atf5-/- P0幼鼠体内分离鼻中隔和鼻甲，每一种基因型取3份样本并混合为一个单样本；每种基因型共制备3份独立样本。