NSD3-Short Represses E-cadherin Expression in A549 Lung Epithelial Cancer Cells

SGC Open Notebook Project to Characterize the HMTase NSD3

Exp024 Objective: In a previous experiment (exp023), we show that siRNA-mediated knockdown of NSD3 increases expression of E-cadherin, a marker of epithelial cell identity. To determine which isoform of NSD3 is involved in promoting epithelial to mesenchymal transition, I have designed siRNA that targets either the long or short isoform and again use E-cadherin expression as a marker. Additionally, I have started using A549 lung epithelial cancer cells (https://www.atcc.org/Products/All/CCL-185) as my primary model. This cell line proliferates faster and is more amenable to in vitro experimentation than the H1299 cell line I was using previously.