Investigation of long non-cording RNAs involved in innate immunity in the acute phase of Kawasaki disease

Kawasaki disease (KD) is a systemic vasculitis that is the most common cause of acquired heart disease in children. Its vasculitis sometimes results in the disruption of the coronary artery structure, which leads to coronary artery aneurysms. Non-coding RNAs are non-protein-coding transcripts, and long non-coding RNAs (lncRNA) are classified as those longer than 200 nucleotides. LncRNAs contribute to the pathophysiology of various diseases. The goal of this project is to investigate whether there are lncRNAs involved in the acute phase of KD. Patients with KD who responded to intravenous immunoglobulin (IVIG) treatment and whose fever diminished within 48 hours of treatment were designated as responders, while patients with KD who did not respond to IVIG were designated non responder patients. Blood samples were collected before and after treatment for each group of responders and non-responders of KD patients. We collected three cases respectively. We also collected samples of two patients with coronary artery disease (CAL). Additionally, three healthy control blood samples were also collected . Only monocytes were separated from blood samples and RNA was extracted. The expression level of lncRNAs and cording RNAs in monocytes were determined by Cap analysis gene expression sequencing (CAGE-seq).