The effect of inactivated bacteria on the redox status of larvae of the wax moth Galleria mellonella

Insects’ defenses can be directed at both live and inactivated pathogens. Recognition of pathogen-associated structures leads to modulation of innate immune responses and oxidative stress in the host. The article presents results of experiments showing that inactivated vegetative cells of Bacillus thuringiensis, B. subtilis, and B. cereus can modulate immune responses in Galleria mellonella in the absence of damage to tissues of the midgut. In the first hours after feeding on Bacillus spp., an increase in the number of immunocompetent hemocytes was observed. Phenoloxidase activity in the hemolymph of wax moth larvae was inhibited, which is confirmed by the suppression of the production of reactive oxygen species in hemolymph. In addition, it was found that the antioxidant response to the bacterial inoculation in the midgut and hemolymph is different. Probably, the local antioxidant defense system is probably configured in such a way that the level of reactive oxygen species increases ra..., , # Data from: The effect of inactivated bacteria on the redox status of larvae of the wax moth Galleria mellonella

Dataset DOI: [10.5061/dryad.jdfn2z3nz](10.5061/dryad.jdfn2z3nz)

## Description of the data and file structure

Data set for the manuscript by Jana L. Vorontsova et al. “Effect of inactivated bacteria on the redox status of *Galleria mellonella* wax moth larvae”, including

(i) survival of *G. mellonella* wax moth larvae after *per os* administration of inactivated vegetative cells of *B. thuringiensis*, *B. subtilis* or *B. cereus*,

(ii) total hemocyte count (THC) (a) and phenoloxidase (PO) activity (b) in the hemolymph of *G. mellonella* wax moth larvae 12 and 24 h after *per os* administration of inactivated vegetative cells of *B. thuringiensis*, *B. subtilis* or *B. cereus*,

(iii) the magnitude of reactive oxygen species (ROS) production (a, c) and total antioxidant activity (TAC) (b, d) in the hemolymph (a, b) and midgut (c, d) of *G. mellonella* wax moth larvae 12 ...,