Stephen Smith, 2011 Olympus BioScapes Digital Imaging Competition® (2012) CIL:41403, Mus musculus, neuron. CIL. Dataset

Video of an array tomogram of a mouse somatosensory cortex. The volume is a "fly-through" of a sagitally oriented rectangular slab of transgenic mouse somatosensory cortex. The overlay thumbnail at upper left renders a projection through the entire slab and the moving magenta rectangle shows the variably smaller area rendered full screen. The rendered volume was acquired by array tomography and stitched together in three dimensions from >10,000 image tiles. Green in this movie renders native FFP fluorescence, red renders anti-synapsin 1 immunofluorescence, and blue renders anti-tubulin immunofluorescence. Dr. Smith wishes to acknowledge the work of Kristina Micheva, Brad Busse, Nicholas Weiler, Nancy O'Rourke and Gordon Wang. This video does not have audio on the Olympus BioScapes website, but a score by Catherine Rose Smith has been composed and performed to accompany the video. More information is provided in two articles: Micheva, K.D., Busse, B.L., Weiler, N.C., O'Rourke, N., Smith, S.J (2010), Neuron 68:639-653; and Micheva, K.D., and Smith, S.J (2007), Neuron 55:25-36. Judges' Special Award for Technical Merit, 2011 Olympus BioScapes Digital Imaging Competition®.

本数据集展示了一只小鼠体感皮层的阵列断层扫描视频。该体积数据呈现为对转基因小鼠体感皮层纵向排列矩形切片的“飞行”视角。左上角的叠加缩略图显示了整个切片的投影，而移动的粉红色矩形展示了依次缩小的全屏渲染区域。所渲染的体积数据通过阵列断层扫描获得，并从超过10,000个图像碎片中在三维空间中进行拼接。视频中绿色部分呈现了原生的FFP荧光，红色部分呈现了抗突触蛋白1免疫荧光，蓝色部分呈现了抗微管蛋白免疫荧光。史密斯博士愿意对克里斯蒂娜·米切瓦、布拉德·布斯、尼古拉斯·维勒、南希·奥鲁克和戈登·王的研究工作表示认可。此视频在Olympus BioScapes网站上未包含音频，但凯瑟琳·罗斯·史密斯为其创作并演奏了配乐。更多详细信息请参阅以下两篇文章：Micheva, K.D., Busse, B.L., Weiler, N.C., O'Rourke, N., Smith, S.J (2010), Neuron 68:639-653；以及Micheva, K.D., and Smith, S.J (2007), Neuron 55:25-36。本视频荣获2011年Olympus BioScapes数字成像竞赛技术卓越特别奖。