mRNA-seq in human 293T HEK cells in the presence (shScr) or absence of HSF1 (shHSF1) or CTCF (shCTCF).

Profiling the mRNA content in 293T human embryonic kidney (HEK) cells in the presence and absence of HSF1 or CTCF. The mRNA was enriched from total cellular RNA using using polyadenyltion-selection. HSF1 (shHSF1) or CTCF (shCTCF) was downregulated using shRNA for 48hours. Scrambled RNA (shScr) was used as a negative control. Paired-end sequencing of mRNA content in 293T human embryonic kidney (HEK) cells. The mRNA was enriched from total cellular RNA of 293T HEK after transfecting the cells with shRNA against either a scrambled RNA (shScr; negative control), HSF1 (shHSF1; HSF1down-regulation), or CTCF (shCTCF; CTCF downregulation). The samples were prepared in four biological replicates.