Kiatmetha_Raw Data 3&4

Figure 3D: Agarose gel electrophoresis of the encapsidated EGFP-plasmid DNA into the VLP interior showing a major DNA band at 3.1 kbp (lane 4). An additional minor band at 6.2 kbp (lane 3; arrowheads) of unloaded plasmid was also noted. Figure 4A: Agarose gel electrophoresis of EGFP gene (600 bp product) present in the shrimp muscle, gill and hepatopancreas.Figure 4B: Confocal microscopy of shrimp tissues injected with EGFP-IHHNV-VLPs (green), and stained with anti-GFP (red). The bottom-most row represents control where the shrimp are injected with unloaded IHHNV-VLPs.Figure 4C: Western blot showing anti-GFP reactivity in the EGFP-IHHNV-VLPs injected shrimp's tissues.