Reversible dietary effect on intestinal stem cells

We report the application of bulk RNAseq assay in examining the dietary effect on mouse intestinal stem cell (ISC) subpopulation. Lgr5EGFPcreERT2 mice were fed with new western diet 1 (NWD1) or the control AIN76A diet for 3 or 12 months, to investigate differential dietary effect. To examine the reversibility of dietary effect, after 3 months of NWD1 feeding, the mice were switched to AIN feeding, and examined at 6 or 12 months old. Overall design: Examine differential dietary effect on the transcriptome profile of ISC cells After weaning, Lgr5EGFPcreERT2 mice were fed with AIN or NWD1 for 3 or 12 months and subjected to RNAseq examination. For Crossover experiments, after 3 months feeding of NWD1, the diet was switched to AIN, and mice were examined in 6 months or 12 months.