Transcriptome analysis in transgenerational epigenetic inheritance

The study was performed towards understanding transgenerational epigenetic inheritance at transcriptome level. The founder males of F0 generation (actin-GAL4/Pin; UASRNAi-mts/+), and the resulting, male-line derived F1 (Pin/+; +/+), and F2 (+/+; +/+) generation individuals were used as test lineage, and the wild-type w1118 progeny as control lineage flies. The fly strains used for generating founder F0 line included Pin/CyO-dYFP; UASRNAi-mts/MKRS, actin-GAL4/CyO; +/+, and w1118. Precursor strains, as appropriate, were outcrossed with w1118 to minimize genetic background differences. F0 males (actin-GAL4/Pin; UASRNAi-mts) were mated with w1118 females to obtain F1 progeny (Pin/+; +/+), and F1 males mated with w1118 females to obtain F2 progeny (+/+; +/+) in the test lineage. In parallel, w1118 flies were self-crossed, and the control lineage flies obtained. Three to five biological replicates were used.