Hemi-methylated CpG sites connect Dnmt1-knockdown-induced and Tet1-induced DNA demethylation during somatic cell reprogramming
收藏干细胞与再生医学数据中心2021-12-14 更新2024-03-06 收录
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资源简介：
Hemimethylation CpG sites generated during cell proliferation are enriched at core pluripotency sites, and Tet1 or sh-RNA against Dnmt1 (sh-Dnmt1)-induced DNA demethylation is enriched at these sites, which, in combination with Yamanaka factors, leads to the upregulation of these genes and promotes somatic cell reprogramming. Moreover, vitamin C was used to further increase the demethylation capacity of TET1 during reprogramming. Therefore it is needed to obtain transcriptome or DNA methylation modification sequencing files by overexpressing or knocking down Tet1CD, Dnmt1, P53 and other genes during reprogramming to further understand the specific mechanisms regarding DNA demethylation during somatic cell reprogramming.
提供机构：
中国科学院广州生物医学与健康研究院
创建时间：
2021-12-14
