Molecular mechanisms of CD4+ T-helper signals required for functional AdV-specific memory CTL responsesa.

aOne day prior to immunization, MHCII−/− mice were adoptively transferred with CD11c+ DCs (∼0.5–1.0×106/mouse) and naïve polyclonal (∼15–20×106/mouse) or OTII CD4+ T cells (∼1.5×106/mouse) with or without designated gene deficiency, as indicated. 120 days later, all the immunized mice were challenged with BL6-10ova tumor cells. Twenty-four days after the challenge, lung tumor colonies were counted and graded. The data are cumulative of two independent experiments, each comprising five to six mice per group.