Bach2 deficiency leads to spontaneous expansion of IL-4-producing T follicular helper cells and autoimmunity

The transcription factor Bach2 is a critical negative regulator of Tfh cell differentiation, especially IL-4 subset. Tfh cells from the mesenteric lymph nodes of WT and Bach2 CD4 conditional KO mice were collected to process the Rna-seq. Mechanistically, Bach2 may limit abnormal IL-4-produicng Tfh cell formation by repressing c-Maf, CXCR5 and IL-4. 10000-30000 Tfh Cells (CD4+B220-CD44+PD-1+CXCR5+) were sorted from the MLNs of WT and Bach2fl/flCd4-cre mice. cDNA was synthesized and amplified using the SMARTer Ultra Low RNA Kit for Illumina Sequencing (Clontech). The library for mRNA sequencing was generated using Illumnia TruSeq Preparation Kit (RS-122-2001) and sequenced using Illumnia Nextseq500 sequencer.