Removal of Spo11 and Resection of 5' Ends of DNA

After cleaving DNA Spo11 remains covalently attached to the 5' ends. Spo11 is removed by cleavage of the DNA and release of Spo11:oligonucleotide complexes (Neale et al. 2005, Pan and Keeney 2009). In yeast, the Mre11:Rad50:Xrs2 complex is required for processing of double-strand breaks introduced by Spo11 (Cherry et al. 2007). The homologous Mre11:Rad50:Nbs1 complex from mouse has not been observed to localize to double-strand breaks made by Spo11 on autosomes. The enzymes responsible for removal of Spo11 and resection of the 3' ends remain unknown.

在DNA的裂解过程中，Spo11酶依然以共价键的形式附着于5'端。Spo11酶通过DNA裂解及Spo11:寡核苷酸复合物的释放而被去除（Neale等，2005年；Pan和Keeney，2009年）。在酵母中，Mre11:Rad50:Xrs2复合体对于由Spo11引入的双链断裂的处理至关重要（Cherry等，2007年）。来自小鼠的同源Mre11:Rad50:Nbs1复合体尚未观察到定位于由Spo11在常染色体上形成的双链断裂处。负责去除Spo11并切除3'端酶的具体身份尚属未知。