Gene expression profile at single cell level of HIV-1 and HIV-2 specific CD8 T cells

CD8 T cells play a crucial role in controlling HIV infection. We employed single-cell RNA sequencing (scRNAseq) to analyze HIV-1 specific CD8 T cells after years of treated infection. Additionally, HIV-2 specific CD8 T cells were studied to serve as a control for an effective anti-HIV response. HIV-specific CD8 T cells from all patients at each time point were index-sorted by FACS into 96-well microtiter plates for scRNAseq analysis.