Expression data from 1-week-old mouse liver

mRNA degradation critically contributes to liver development and function. The CCR4-NOT complex serves as a major deadenylase that initiates mRNA degradation. We used microarrays to identify deregulated genes in the livers lacking CNOT3, a core subunit of the CCR4-NOT complex. Liver-specific CNOT3 knockout mice were generated by expressing Cre recombinase under the control of Albumin promoter. Livers from control and CNOT3 knockout mice were used for RNA extraction and hybridization on Affymetrix microarrays.信使核糖核酸（mRNA）的降解在肝脏发育与功能维持中发挥关键作用。CCR4-NOT复合物是启动mRNA降解的主要脱腺苷酸化酶（deadenylase）。我们利用基因芯片（microarrays）技术，筛选缺失CCR4-NOT复合物核心亚基CNOT3的肝脏组织中表达失调的基因。通过在白蛋白启动子（Albumin promoter）调控下表达Cre重组酶（Cre recombinase），我们构建了肝脏特异性敲除CNOT3的小鼠模型。我们分别提取对照组与CNOT3敲除小鼠的肝脏组织RNA，并将其与Affymetrix基因芯片进行杂交实验。