c-kitCre Knock-ins Fail to Identify, Effectively Recombine and Fate-Map Cardiac Stem Cells
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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A recent cell-fate mapping study by Van Berlo et al. using a Cre knock-in (KI) into the c-kit locus, and two other studies using a similar genetic approach to track the fate of Cardiac Stem/progenitor Cells (CSCs) concluded that c-kitpos cells only negligibly generate cardiomyocytes. These papers questioned our findings that tissue-specific c-kitposCSCs are endogenous regenerative agents necessary and sufficient for cardiomyocyte regeneration/replenishment after injury. We consider answering whether the c-kitCre-KI approach properly identifies and fate-maps c-kitposCSCs and/or whether c-kitCre-KI-insertion affects CSC biology and cardiomyogenic potential. To test this hypothesis, we crossed the the c-kitCre-KI mouse to different reporter mouse models. We concluded that the c-kitCre-KI approach neither identifies nor fate-tracks the c-kitposCSCs, because c-kit expression level in these cells is too low to produce enough Cre to effectively recombine the floxed marker and permanently tag the CSC and their progeny.
提供机构：
Magna Graecia University
创建时间：
2022-02-20
