Transcriptome profiling of senescent and non-senescent BPH epithelial cells after CD4? T cell co-culture

Human primary CD4? T cells were co-cultured with either senescent or non-senescent primary BPH epithelial cells for 6 hours. BPH epithelial cells were then isolated and subjected to RNA sequencing. The experiment was repeated three times independently. Overall design: Primary human CD4? T cells were co-cultured for 6 hours with senescent or non-senescent BPH epithelial cells, which were pre-identified by SA-ß-gal staining. Following co-culture, epithelial cells were isolated and subjected to transcriptome profiling (bulk RNA-seq). The study included three biological replicates per group.