High-Low Fat Diet/Strain Experiment

Males and females from each SM/J and LG/J were placed on either a low-fat diet or high-fat diet immediately after weaning until 20 weeks of age. At 20 weeks, mice were sacrificed in late morning after a four-hour fast and liver tissue was collected from 4 males and 4 females from each strain and diet.将SM/J与LG/J两个品系的雌雄小鼠，于断奶后即刻分别饲喂低脂饮食（low-fat diet）或高脂饮食（high-fat diet），直至20周龄。待小鼠长至20周龄时，在禁食4小时后的上午晚些时候将其处死，并从每个品系及对应饮食组的4只雄性与4只雌性小鼠体内采集肝脏组织。