Custom gene expression panel for evaluation of potential molecular markers in hepatocellular carcinoma

We studied 20 candidate genes (HCC hub genes, potential drug target genes, predominant somatic mutant genes) retrieved from available literature and public databases with potential to be used as biomarkers for the prognosis of HCC and prediction of treatment outcome. We analysed expression of the genes by RT-qPCR in 30 HCC tumor and adjacent non-tumor paired samples from Vietnamese patients. qPCR gene expression profiling. Thirty Hepatocellular carcinoma (HCC) tumor and adjacent non-tumor paired samples were used in this study. One microgram (1µg) total RNA from each sample was used for gene expression analysis.